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KMID : 1004820120130020141
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Journal of Biomedical Research
2012 Volume.13 No. 2 p.141 ~ p.147
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Genetic Identification of Subspecies and Stocks of Minke Whale Meats Purchased from Korean Markets during 2005-2007
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Koh Hung-Sun
Jang Kyung-Hee
Han Eui-Dong
Jo Jae-Eun
Jeong Seon-Ki
Ham Eui-Jeong
In Seong-Taek
Lee Jong-Hyek
Kweon Gu-Hee
Kim Kwang-Seon
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Abstract
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To identify subspecies and stocks of minke whale meats purchased from Korean markets during 2005-2007, we first obtained their complete sequences of mitochondrial DNA cytochrome b and control region sequences, and compared these sequences to the corresponding sequences of the common minke whale (Balaenoptera acutorostrata), obtained from GenBank. From analyses with partial cytochrome b sequences (383 bp) and non-coding, partial control region sequences (463 bp), Korean mink whale meats are identified as products from the North Pacific minke whale (B. a. scammoni). In addition, the sequences of the partial control region from these meats showed G at site no. 298 and G or A at site no. 463, and the meats appeared to originate from the J stock within this subspecies. Thus, because the J stock has been protected since 1986, implementation of strict regulation measures to reduce their accidental fisheries by catch seems urgent. In addition, B. a. scammoni is distinct from B. a. acutorostrata, with an average Jukes-Cantor distance of 2.21% in the complete control region sequence analysis (935 bp) and 1.31% in the complete cytochrome b gene sequence analysis; the current results support the current subspecies classification, although further sequencing analyses with nuclear genes are necessary.
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KEYWORD
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Molecular identification, Korean minke whale meats, cytochrome b and control region sequences, Balaenoptera acutorostrata scammoni, J-stock
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